Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

Senamela, Tshiphiri; Kock, Marleen M.; Becker, Piet J.; Potgieter, Joachim

Please note that UPSpace will be unavailable from Friday, 2 May at 18:00 (South African Time) until Sunday, 4 May at 20:00 due to scheduled system upgrades. We apologise for any inconvenience this may cause and appreciate your understanding.

Show simple item record

dc.contributor.author	Senamela, Tshiphiri
dc.contributor.author	Kock, Marleen M.
dc.contributor.author	Becker, Piet J.
dc.contributor.author	Potgieter, Joachim
dc.date.accessioned	2019-04-17T09:00:10Z
dc.date.available	2019-04-17T09:00:10Z
dc.date.issued	2018-01-31
dc.description.abstract	The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.	en_ZA
dc.description.department	Haematology	en_ZA
dc.description.librarian	am2019	en_ZA
dc.description.sponsorship	The National Health Laboratory Service research trust	en_ZA
dc.description.uri	http://www.ajlmonline.org	en_ZA
dc.identifier.citation	Senamela T, Kock M, Becker P, et al. Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay. African Journal of Laboratory Medicine 2018;7(1), a658. https://DOI.org/10.4102/ajlm.v7i1.658.	en_ZA
dc.identifier.issn	2225-2002 (print)
dc.identifier.issn	2225-2010 (online)
dc.identifier.other	10.4102/ajlm.v7i1.658
dc.identifier.uri	http://hdl.handle.net/2263/68993
dc.language.iso	en	en_ZA
dc.publisher	AOSIS Open Journals	en_ZA
dc.rights	© 2018. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.	en_ZA
dc.subject	Diagnostic laboratories	en_ZA
dc.subject	Sequencing	en_ZA
dc.subject	Polymerase chain reaction (PCR)	en_ZA
dc.subject	Detection	en_ZA
dc.subject	Janus kinase 2 V617F mutation	en_ZA
dc.subject.other	Health sciences articles SDG-03
dc.subject.other	SDG-03: Good health and well-being
dc.subject.other	Health sciences articles SDG-09
dc.subject.other	SDG-09: Industry, innovation and infrastructure
dc.subject.other	Health sciences articles SDG-17
dc.subject.other	SDG-17: Partnerships for the goals
dc.title	Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay	en_ZA
dc.type	Article	en_ZA