Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

Senamela, Tshiphiri; Kock, Marleen M.; Becker, Piet J.; Potgieter, Joachim

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Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

Senamela, Tshiphiri; Kock, Marleen M.; Becker, Piet J.; Potgieter, Joachim

URI: http://hdl.handle.net/2263/68993

Date: 2018-01-31

Abstract:

The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.

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