Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin

Mbuayama, Kabuzi R.; Taute, Helena; Stromstedt, Adam A.; Bester, Megan Jean; Gaspar, Anabella Regina Marques

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Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin

Mbuayama, Kabuzi R.; Taute, Helena; Stromstedt, Adam A.; Bester, Megan Jean; Gaspar, Anabella Regina Marques

URI: https://repository.up.ac.za/handle/2263/86414

Date: 2022-05

Abstract:

Please read abstract in the article.

Description:

Supporting Information: Table S1. Physicochemical properties and antifungal activity of OsDef2 derived peptides. Figure S1. Effect of sodium azide on the intracellular translocation of 5-FAM penetratin. C. albicans cells were pre-incubated with 5mM NaN3 for 30 min and treated with 5-FAM penetratin. The cells were counterstained with DAPI (blue) and visualized by confocal microscopy. Scale bars are 10 μm. Figure S2. DAPI stained images of 5-FAM-peptides in C. albicans. Cells were exposed for 50 min to 2.5 μM of 5-FAM-peptides, counterstained with DAPI (blue) and visualized by confocal microscopy. Top and bottom panel show same images at lower and higher magnification, respectively. Short arrow: fragmented nuclear DNA; long arrow: tubular-shaped DNA, in contrast to single and round nucleus observed in untreated cells. Images are representatives of three independent experiments, in duplicate. Scale bars are 10 μm.

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