Clofazimine exposure in vitro selects efflux pump mutants and bedaquiline resistance

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Clofazimine exposure in vitro selects efflux pump mutants and bedaquiline resistance

Ismail, Nabila; Peters, Remco P.H.; Ismail, Nazir Ahmed; Omar, Shaheed Vally
URI: http://hdl.handle.net/2263/68613
Date: 2019-03

Abstract:

Six in vitro clofazimine-resistant spontaneous mutants obtained from a wild-type or pyrazinamide-resistant ATCC reference strain were selected to evaluate bedaquiline cross-resistance. The reverse was conducted for bedaquiline mutants. All clofazimine mutants harboring an rv0678 mutation displayed phenotypic cross-resistance. We observed the same for rv0678 bedaquiline mutants; however, atpE bedaquiline mutants showed no phenotypic cross-resistance. This confirms that upfront clofazimine usage may impact subsequent bedaquiline use due to a shared efflux resistance pathway.

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