An accurate, sensitive and reproducible method for colorimetric estimation of free fatty acids in plasma
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| dc.contributor.author | De Villiers, Saramarie
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| dc.contributor.author | Van der Walt, J.G.
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| dc.contributor.author | Procos, J.
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| dc.contributor.editor | Bigalke, R.D. | |
| dc.contributor.editor | Cameron, Colin McKenzie | |
| dc.contributor.editor | Gilchrist, Frances M.C. | |
| dc.contributor.editor | Morren, A.J. | |
| dc.contributor.editor | Verster, Anna J.M. | |
| dc.contributor.editor | Verwoerd, Daniel Wynand | |
| dc.contributor.editor | Walker, Jane B. | |
| dc.contributor.other | De Kock, V.E. | |
| dc.date.accessioned | 2016-07-06T11:09:31Z | |
| dc.date.available | 2016-07-06T11:09:31Z | |
| dc.date.created | 2016 | |
| dc.date.issued | 1977 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en_ZA |
| dc.description.abstract | A sensitive and reproducible method permitting the estimation of 0,030-3,000 mM of free fatty acids (FFA) in plasma with a standard error of 0,006 5 was developed. The method is based on the extraction of phosphate-buffered plasma with a chloroform-heptane-methanol mixture, formation of the cobalt complex and on the subsequent determination of the metal with 1-nitroso-2 naphthol. The method was used to obtain normal values (mean 0,547 mM range 0,147-1,197 mM) for Merino wethers fed lucerne hay. | en_ZA |
| dc.identifier.citation | De Villiers, S, Van der Walt, JG & Procos, J 1977, 'An accurate, sensitive and reproducible method for colorimetric estimation of free fatty acids in plasma’, Onderstepoort Journal of Veterinary Research, vol. 44, no. 3, pp. 169-172. | en_ZA |
| dc.identifier.issn | 0330-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/53722 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | Pretoria : The Government Printer | en_ZA |
| dc.rights | © 1977 ARC – Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © 2016 University of Pretoria. Department of Library Services (digital). | en_ZA |
| dc.subject | Veterinary medicine | en_ZA |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.title | An accurate, sensitive and reproducible method for colorimetric estimation of free fatty acids in plasma | en_ZA |
| dc.type | Article | en_ZA |
