Browsing 1977 Onderstepoort Journal of Veterinary Research, Volume 44, 1977 by Issue Date

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Browsing 1977 Onderstepoort Journal of Veterinary Research, Volume 44, 1977 by Issue Date

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  • Negative staining of a non-haemadsorbing strain of African swine fever virus 
    Els, H.J.; Pini, A.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    Since the application of negative staining, preceded by fixation, prevents the disruption and distortion of the capsid of the African swine fever virus, improved contrast and evaluation of the appearance and size of virus ...
  • The life cycle of the two-host tick Rhipicephalus evertsi evertsi Neumann, 1897, under laboratory conditions (Acarina : Ixodidae) 
    Londt, J.G.H.; Van der Bijl, Eleanore B.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    The life cycle of Rhipicephalus evertsi evertsi Neumann, 1897, is discussed under the following headings: larval feeding; larval drop; nymphal feeding; nymphal drop; nymphal moulting; adult feeding; adult drop; preoviposition ...
  • Amaurosis in sheep resulting from treatment with rafoxanide 
    Prozesky, Leon; Pienaar, J.G.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    Amaurosis occurred in sheep on various farms in the Republic of South Africa after treatment with rafoxanide. Histopathological examination revealed a status spongiosus of varying severity in the central nervous system ...
  • Neurosecretion in Ornithodoros savignyi (Audouin) (Ixodoidea : Argasidae). The distribution of neurosecretory cells in the brain 
    Evans, Aileen A.; Solomon, K.R.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    The arrangement of the brain and peripheral nerves in Ornithodoros savignyi (Audouin) is similar to that of other argasid and ixodid ticks. Histological studies, using a specialized staining technique (aldehyde fuchsin), ...
  • Studies on Schistosomiasis. 9. Pathology of the bovine urinary tract 
    Bartsch, R.C.; Van Wyk, Jan Aucamp; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    Pathological findings in the urinary tract of 40 head of cattle, experimentally or naturally infested with Schistosoma mattheei, are presented. Lesions of the ureter found in 57,5% of the cases consisted of linear granulomata ...
  • Parasites of domestic and wild animals in South Africa. III. Oestrus spp. and Gedoelstia hässleri in the blesbok 
    Horak, Ivan Gerard; Butt, M.J.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    Four blesbok culled in the Rietvlei Nature Reserve, Pretoria, District during May 1972 were found to harbour large burdens of 1st instar Oestrus spp. larvae and from 16-37 3rd instar larvae of Oestrus macdonaldi. They were ...
  • Gamma-glutamyl transpeptidase activity in sheep serum : normal values and an evaluation of its potential for detecting liver involvement in experimental lupinosis 
    Malherbe, W.D.; Kellerman, T.S.; Kriek, N.P.J.; Haupt, W.H.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    A brief survey of the literature on gamma-glutamyl transpeptidase (ƴ-GT) activity is included in this study. The levels of activity in the serum of normal Merino sheep (13, 6- 32, 4 mI.U./ml) were ascertained as a preliminary ...
  • Black grain mycetoma (maduromycosis) in horses 
    Coetzer, Jacobus A.W.; Boomker, Jacob Diederik Frederik; Scott, D.B.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    Black grain mycetoma occurred in two horses, one a cross-bred pony and the other a Thoroughbred racehorse. Lesions were limited to wart-like growths in one case and a deep-seated girth gall in the other. In both cases the ...
  • The immunological response to intact and dissociated bluetongue virus in mice 
    Carter, P.; Oellermann, R.A.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    Antigenic fractions of bluetongue virus were separated by ultracentrifugation in Tris-buffered CsCl gradients at pH 6, 7 or 8 and the bluetongue virus polypeptide composition of the bands isolated from these gradients was ...
  • Oviposition and incubation in Boophilus decoloratus (Koch, 1844) (Acarina : Ixodidae) 
    Londt, J.G.H.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    Studies on the duration of the oviposition and incubation periods of Boophilus decoloratus (Koch, 1844) revealed that these non-parasitic periods are temperature dependent, increase in temperature causing shortening of the ...
  • Parasites of domestic and wild animals in South Africa. I. Oestrus ovis in sheep 
    Horak, Ivan Gerard; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    Separate groups of 3 oestrid-free lambs were exposed to infestation on irrigated pasture for periods of approximately 33 days each over 30 months, and on dry-land pasture for approximately 42 days over a period of 18 months. ...
  • The separation of some volatile fatty acids on a "Sephadex" partition chromatogram 
    Van der Walt, J.G.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer,Pretoria, 1977)
    The suitability of Sephadex LH-20 as a solid support for the partition chromatography of acetic, propionic and butyric acids was investigated by using standard mixtures of 14C-labelled acids. The clear separation that was ...
  • Studies on Parafilaria bovicola Tubangui, 1934 : 2. Chemotherapy and pathology 
    Viljoen, J.H.; Boomker, Jacob Diederik Frederik; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    The filaricidal effects of trichlorphon, arsenic trioxide, sodium antimony biscathecol disulphonate and nitroxynil against Parafilaria bovicola were the subject of this investigation . Levamisole hydrochloride was retested ...
  • The fine structure of intra-erythrocytic stages of Babesia bigemina 
    Potgieter, F.T.; Els, H.J.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    The electron microscope was used to study the structure of merozoites, merozoites in the process of transformation to trophozoites, trophozoites, and the method of multiplication of B. bigemina. The merozoites were piriform ...
  • Sheep erythrocyte and bluetongue virus antibody responses of spleen cell cultures from mice 
    Carter, P.; Oellermann, R.A.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Government Printer, Pretoria, 1977)
    The optimum conditions for the culture of cells from dissociated spleens were determined. Routinely, 10⁷ cells were seeded per ml of RPMI 1640 medium supplemented with 20% pre-tested foetal calf serum. For the assay of the ...
  • Transplantation of cultured jaagsiekte (sheep pulmonary adenomatosis) cells into athymic nude mice 
    Du Plessis, J.L.; Verwoerd, Daniel Wynand; Meyer-Scharrer, Elizabeth; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    Epithelial cells of the 15 .4 line, which were originally established from the adenomatous lung of a jaagsiekte case and which had been cultured in vitro for 22 generations, were injected subcutaneously into athymic nude ...
  • Disseminated protothecosis in a dog 
    Imes, G. D.; Lloyd, June C.; Brightman, M. P.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    This is a report on the clinical history and pathological lesions of a dog suffering from disseminated protothecosis due to Prototheca zopfii. Clinically, the dog was presented with bilateral conjunctivitis followed by ...
  • Subject index 
    Anonymous; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
  • A rapid method for differentiating between the infective larvae of Oesophagostomum columbianum and Chabertia ovina 
    Van Wyk, Jan Aucamp; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    When freshly-collected, exsheathed infective larvae of Oesophagostomum columbianum and Chabertia ovina were frozen in liquid nitrogen and subsequently thawed, conspicuous vesicles appeared in the intestinal cells of the ...
  • Failure to induce in rabbits effective immunity to a mixed infection of Fusobacterium necrophorum and Corynebacterium pyogenes with a combined bacterin 
    Cameron, Colin McKenzie; Fuls, W.J.P.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    Rabbits were immunized with alum-precipitated, oil adjuvant and an untreated bacterin composed of F. necrophorum and C. pyogenes. Immunized rabbits were challenged intradermally with a mixture of F. necrophorum and C. ...