Rapid molecular strategy for filovirus detection and characterization

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Rapid molecular strategy for filovirus detection and characterization

Zhai, Junhui; Palacios, Gustavo; Towner, Jonathan S.; Jabado, Omar; Kapoor, Vishal; Venter, Marietjie; Grolla, Allen; Briese, Thomas; Paweska, Janusz Tadeusz; Swanepoel, Robert; Feldmann, Heinz; Nichol, Stuart T.; Lipkin, Ian W.
URI: http://hdl.handle.net/2263/4328
Date: 2007-01

Abstract:

Filoviruses have the capacity to cause lethal outbreaks of hemorrhagic fever in primates. Here we present a simple consensus reverse transcription-PCR method for filovirus recognition and characterization and demonstrate its utility with all known filovirus strains. Phylogenetic assignment is achieved by automated web-based sequence analysis of amplification products.

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