Browsing 2002 Onderstepoort Journal of Veterinary Research, Volume 69, 2002 by Issue Date

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Browsing 2002 Onderstepoort Journal of Veterinary Research, Volume 69, 2002 by Issue Date

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  • The Kumm isolate of Ehrlichia ruminantium: in vitro isolation, propagation and characterization 
    Zweygarth, Erich; Van Heerden, Henriette; Allsopp, Basil A.; Josemans, A.I.; Van Strijp, M.F.; Allsopp, M.T.E.P.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An effective culture system for Ehrlichia (Cowdria) ruminantium comb. nov. was first established in 1985 and many stocks were subsequently isolated and propagated in vitro. A notable exception, however, was the Kumm isolate ...
  • The effect of three bufadienolide cardiac glycosides on contraction of isolated rat jejunum 
    Botha, C.J. (Christoffel Jacobus); Gehring, Ronette; Van Rooyen, J.M.; Venter, D.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Three cardiac glycosides were screened for pharmacological effects on isolated rat jejunum. The contraction of rat jejunum with epoxyscillirosidin, a non-cumulative bufadienolide, and cotyledoside and tyledoside D, both ...
  • Seasonal abundance of adult ixodid ticks infesting cattle belonging to resource-limited farmers in the north-eastern Free State Province of South Africa 
    Hlatshwayo, M.; Mbati, P.A.; Dipeolu, O.O.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A study was undertaken to determine the seasonal abundance of the ticks infesting cattle owned by resource-limited farmers in the northeastern Free State Province of South Africa. Infestations of Boophilus decoloratus, ...
  • Review of current problems and shortcomings in the Tanzanian animal health information system with suggestions on improvement 
    Kivaria, F.M.; Kapaga, A.M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Livestock diseases have always been the focus of the Tanzanian Veterinary Authorities. However, they have become more important since the formation of the World Trade Organisation and subsequent implementation of the various ...
  • Virulence of South African isolates of Haemophilus paragallinarum. Part 2: Naturally occurring NAD-independent field isolates 
    Bragg, R.R. (Robert Richard); Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Naturally occurring NAD-independent variants of Haemophilus paragallinarum, which have been isolates from poultry showing clinical signs of infectious coryza, were used to determine their virulence using a newly developed ...
  • Acaricide resistance profiles of single- and multi-host ticks from communal and commercial farming areas in the Eastern Cape and North-West Provinces of South Africa 
    Horak, Ivan Gerard; Mekonnen, Sileshi; Bryson, N.R.; Fourie, L.J.; Peter, R.J.; Spickett, Arthur M.; Taylor, Robin J.; Strydom, T.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A field study (February 2000 to August 2001) was conducted on communal and commercial farms in the Eastern Cape and North-West Provinces of South Africa to detect the levels of tick resistance to commonly used acaricides. ...
  • Strategic control of gastrointestinal nematodes of sheep in the highlands of central Kenya 
    Maingi, N.; Otieno, R.O.; Gichohi, V.M.; Weda, E.H.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    The effectiveness of anthelmintic treatments given 3 weeks after the onset of rains to control gastrointestinal nematodes in sheep in the highlands of central Kenya was investigated. The study was carried out on a farm ...
  • A survey on the occurrence of resistance to anthelmintics of gastrointestinal nematodes of goats in Mozambique 
    Boomker, Jacob Diederik Frederik; Atanasio, A.; Sitoe, C.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A survey to study the extent of anthelmintic resistance was conducted in Maputo and Gaza, two of the ten provinces of Mozambique, during February and March 1999. A total of 12 flocks, six in Maputo and six in Gaza, was ...
  • Amphistome infection of goats farmed under resource-poor conditions in South Africa 
    Vatta, A.F.; Krecek, R.C.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    From December 1998 to April 2000, a longitudinal study was conducted of the pooled trematode faecal egg counts of samples collected from goats of resource-poor farmers at Rust de Winter, Gauteng Province, Impendle, ...
  • Virulence of South African isolates of Haemophilus paragallinarum. Part 3: Experimentally produced NAD-independent isolate 
    Taole, M.; Albertyn, J.; Van Heerden, E.; Bragg, R.R. (Robert Richard); Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A NAD-dependent isolate 46 (C-3) of Haemophilus paragallinarum, which was previously demonstrated to be of high virulence, was transformed to NAD independence using a plasmid isolated from a naturally occurring NAD-independent ...
  • Development of a diagnostic one-tube RT-PCR for the detection of Rift Valley fever virus 
    Nel, Louis Hendrik; Espach, Anel; Romito, M.; Viljoen, G.J.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Diagnosis of Rift Valley fever (RVF) is based on serology and virus isolation. The disadvantages of the former include poor sensitivity, high cost, risks associated with using infectious virus as antigen, the lengthy ...
  • Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells 
    Sahle, M.; Burgess, W.G.; Kidanemariam, Awoke; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran ...
  • The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa 
    Howell, P.G.; Bosman, Anna-Mari; Coetzer, Jacobus A.W.; Guthrie, Alan John; Groenewald, Daleen; Visage, Carina W.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Selected isolates of equine encephalosis virus were shown to have comparable viral protein profiles and to represent seven distinct serotypes, based on cross-neutralization tests. Serotype-specific virus-neutralizing ...
  • Development of solid phase antigen for indirect ELISA for the detection of specific antibody responses to infection with Newcastle disease virus 
    Sahle, M.; Burgess, G.W.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A simple and inexpensive method of antigen preparation by ultrafiltration was investigated using the V4 strain of Newcastle disease virus. The antigen designated XM300 was used in an indirect enzyme-linked immunosorbent ...
  • Seasonal abundance of four Culicoides spp. (Diptera: Ceratopogonidae) at Al-Ahsa oasis, Eastern Province, Saudi Arabia 
    Abu Elzein, E.M.E.; Hilali, M.; Al-Afaleq, A.I.; Mellor, Philip S.; Boorman, J.; Al-Atiya, S.; Al-Naiem, A.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    This report constitutes the first study of Culicoides spp. and their seasonal abundance at Al-Ahsa, the largest oasis in the Eastern Province of Saudi Arabia. New Jersey light traps were used to collect the midges at Mastock ...
  • Subject index 
    Anonymous; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
  • Non-immune control of trypanosomosis: in vitro oxidative burst of PMA- and trypanosome-stimulated neutrophils of Boran and N'Dama cattle 
    Taiwo, V.O.; Adejinmi, J.O.; Oluwaniyi, J.O.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An in vitro assay that measures the generation of superoxide anions (O₂ˉ) was used to assess the level of oxidative burst of phorbol myristate acetate (PMA) - and trypanosome-stimulated neutrophils isolated from healthy ...
  • The prevalence and economic importance of bovine fasciolosis in Kenya - an analysis of abattoir data 
    Kithuka, J.M.; Maingi, N.; Njeruh, F.M.; Ombui, J.N.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A retrospective study covering a period of 10 years (1990-1999) was carried out using post mortem meat inspection records at the Veterinary Department Headquarters at Kabete to determine the prevalence and economic importance ...
  • Description of Pelonia africana n. g., n. sp. (Pentastomida: Sebekidae) from the lungs of Pelomedusa subrufa and Pelusios sinuatus (Chelonia) in South Africa 
    Boomker, Jacob Diederik Frederik; Junker, Kerstin; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    The terrapins Pelomedusa subrufa and Pelusios sinuatus taken from a water reservoir in the Northern Province, South Africa, were examined for pentastome infections. Two immature specimens, a patent female and a mature male, ...
  • Validation of an indirect enzyme-linked immunosorbent assay for the detection of antibody against Brucella abortus in cattle sera using an automated ELISA workstation 
    Paweska, Janusz Tadeusz; Prozesky, Leon; Potts, A.D.; Harris, H.J.; Smith, S.J.; Viljoen, Gerrit Johannes; Dungu, B.; Brett, O.L.; Bubb, M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An automated indirect enzyme-linked immunosorbent assay (I-ELISA) for the serological diagnosis of bovine brucellosis was developed and validated in-house. A total of 4803 cattle sera from South Africa ( n=3 643), Canada ...