Establishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africa

dc.contributor.advisorVenter, Marietjie
dc.contributor.coadvisorMendes, Adriano
dc.contributor.emailclourens101@gmail.comen_US
dc.contributor.postgraduateLourens, Carla
dc.date.accessioned2023-08-17T06:54:32Z
dc.date.available2023-08-17T06:54:32Z
dc.date.created2023-09-08
dc.date.issued2023
dc.descriptionDissertation (MSc (Medical Virology))--University of Pretoria, 2023.en_US
dc.description.abstractThe burden of WNV and association with neurological disease was identified through syndromic surveillance in animals and humans using available serological tools. WSLB virus was identified in two samples (an equine and a bird) in South Africa for the first time since 2008. Additionally, ELISA’s were developed testing for IgG and IgM antibodies in equines, humans, cattle, and a species-independent assay for WNV and WSLB using the NS1 antigen, which differs from the commercial ELISA’s available that targets the E protein. The human WNV IgM and IgG ELISA showed very promising results, while the equine WNV ELISA’s had quite high background and this could be due to the different target antigen or the use of a baculovirus expressed protein with commercial antibodies. The cattle WNV IgM ELISA also showed good results and can possibly be implemented in the lab. The WSLB ELISA’s were only developed and optimized with rabbit sera, since a clinical sample lacked neutralizing antibodies for use in the assay. Unfortunately, the baculovirus expression of the NS1 protein was unsuccessful.en_US
dc.description.availabilityUnrestricteden_US
dc.description.degreeMSc (Medical Virology)en_US
dc.description.departmentMedical Virologyen_US
dc.description.sponsorshipLEAP-Agri, LEARN (grant number:115574).en_US
dc.description.sponsorshipANDEMIA project (grant number: 01KA1606).en_US
dc.description.sponsorshipG7 Global Health Fund (grant number: ZMVI1-2517GHP703).en_US
dc.description.sponsorshipPoliomyelitis Research Foundation.en_US
dc.description.sponsorshipUniversity of Pretoria Postgraduate bursary.en_US
dc.identifier.citation*en_US
dc.identifier.doi10.25403/UPresearchdata.23968620en_US
dc.identifier.otherS2023
dc.identifier.urihttp://hdl.handle.net/2263/91954
dc.identifier.uriDOI: https://doi.org/10.25403/UPresearchdata.23968620.v1
dc.language.isoenen_US
dc.publisherUniversity of Pretoria
dc.rights© 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
dc.subjectUCTDen_US
dc.subjectSurveillance of arbovirusesen_US
dc.subjectNeurological diseasesen_US
dc.subjectEnzyme-linked immunosorbent assay (ELISA)en_US
dc.subjectSerological assaysen_US
dc.subjectAntibodiesen_US
dc.subject.otherHealth sciences theses SDG-03
dc.subject.otherSDG-03: Good health and well-being
dc.subject.otherHealth sciences theses SDG-08
dc.subject.otherSDG-08: Decent work and economic growth
dc.subject.otherHealth sciences theses SDG-17
dc.subject.otherSDG-17: Partnerships for the goals
dc.subject.otherHealth sciences theses SDG-15
dc.subject.otherSDG-15: Life on land
dc.titleEstablishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africaen_US
dc.typeDissertationen_US

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