HPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination?

dc.contributor.authorBoy, Sonja Catharina
dc.contributor.authorJanse van Rensburg, Estrelita
dc.contributor.authorEngelbrecht, Susan
dc.contributor.authorDreyer, Leonora
dc.contributor.authorVan Heerden, Marlene B.
dc.contributor.authorVan Heerden, Willem Francois Petrus
dc.contributor.emailsonja.boy@up.ac.zaen
dc.date.accessioned2007-06-15T08:24:54Z
dc.date.available2007-06-15T08:24:54Z
dc.date.issued2006-02
dc.description.abstractBackground: High-risk human papilloma viruses (HPV) are reported to be significant independent risk factors for oral squamous cell carcinoma (OSCC). The prevalence of HPV in OSCC in a South African population sample was evaluated comparing three different HPV detection methods. Methods: Tumour and adjacent morphologically normal oral mucosa of 59 resections of primary OSCC were evaluated for the presence of HPV using real-time polymerase chain reaction (PCR), conventional in situ hybridization (ISH), and a signal amplification ISH technique (Dako GenPointTM). Results: HPV18 DNA was detected in seven cases using real-time PCR. No positivity was found with the other two ISH techniques. Conclusions: We support the view that HPV is probably unimportant in the pathogenesis of OSCC and hypothesize HPV detection techniques as the main reason for the positive results in many studies. Real-time PCR was confirmed as the most sensitive technique, but researchers are urged to incorporate strict internal controls when using this detection method.en
dc.format.extent120713 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationBoy, S, Janse van Rensburg, E, Engelbrecht, S, Dreyer, L, Van Heerden, M & Van Heerden, W 2006, 'HPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination?', Journal of Oral Pathology and Medicine, vol. 35, no. 2, pp. 86-90. [http://www.blackwell-synergy.com/loi/jop]en
dc.identifier.issn0904-2512
dc.identifier.other10.1111/j.1600-0714.2006.00385.x
dc.identifier.urihttp://hdl.handle.net/2263/2736
dc.language.isoenen
dc.publisherBlackwellen
dc.rightsThe definitive version is available at www.blackwell-synergy.comen
dc.subjectHuman papillomavirus (HPV)en
dc.subjectIntra-oral squamous cell carcinomaen
dc.subjectOral canceren
dc.subjectReal-time PCRen
dc.subjectOral neoplasmsen
dc.subject.lcshPapillomavirusesen
dc.subject.lcshIn situ hybridizationen
dc.subject.lcshSquamous cell carcinoma (SCC)en
dc.subject.lcshMouth -- Canceren
dc.subject.lcshPolymerase chain reactionen
dc.titleHPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination?en
dc.typePostprint Articleen

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