HPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination?
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Date
Authors
Boy, Sonja Catharina
Janse van Rensburg, Estrelita
Engelbrecht, Susan
Dreyer, Leonora
Van Heerden, Marlene B.
Van Heerden, Willem Francois Petrus
Journal Title
Journal ISSN
Volume Title
Publisher
Blackwell
Abstract
Background: High-risk human papilloma viruses (HPV) are reported to be significant independent risk factors for oral squamous cell carcinoma (OSCC). The prevalence of HPV in OSCC in a South African population sample was evaluated comparing three different HPV detection methods.
Methods: Tumour and adjacent morphologically normal oral mucosa of 59 resections of primary OSCC were evaluated for the presence of HPV using real-time polymerase chain reaction (PCR), conventional in situ hybridization (ISH), and a signal amplification ISH technique (Dako GenPointTM).
Results: HPV18 DNA was detected in seven cases using real-time PCR. No positivity was found with the other two ISH techniques.
Conclusions: We support the view that HPV is probably unimportant in the pathogenesis of OSCC and hypothesize HPV detection techniques as the main reason for the positive results in many studies. Real-time PCR was confirmed as the most sensitive technique, but researchers are urged to incorporate strict internal controls when using this detection method.
Description
Keywords
Human papillomavirus (HPV), Intra-oral squamous cell carcinoma, Oral cancer, Real-time PCR, Oral neoplasms
Sustainable Development Goals
Citation
Boy, S, Janse van Rensburg, E, Engelbrecht, S, Dreyer, L, Van Heerden, M & Van Heerden, W 2006, 'HPV detection in primary intra-oral squamous cell carcinomas – commensal, aetiological agent or contamination?', Journal of Oral Pathology and Medicine, vol. 35, no. 2, pp. 86-90. [http://www.blackwell-synergy.com/loi/jop]