Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

dc.contributor.authorSenamela, Tshiphiri
dc.contributor.authorKock, Marleen M.
dc.contributor.authorBecker, Piet J.
dc.contributor.authorPotgieter, Joachim
dc.date.accessioned2019-04-17T09:00:10Z
dc.date.available2019-04-17T09:00:10Z
dc.date.issued2018-01-31
dc.description.abstractThe purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.en_ZA
dc.description.departmentHaematologyen_ZA
dc.description.librarianam2019en_ZA
dc.description.sponsorshipThe National Health Laboratory Service research trusten_ZA
dc.description.urihttp://www.ajlmonline.orgen_ZA
dc.identifier.citationSenamela T, Kock M, Becker P, et al. Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay. African Journal of Laboratory Medicine 2018;7(1), a658. https://DOI.org/10.4102/ajlm.v7i1.658.en_ZA
dc.identifier.issn2225-2002 (print)
dc.identifier.issn2225-2010 (online)
dc.identifier.other10.4102/ajlm.v7i1.658
dc.identifier.urihttp://hdl.handle.net/2263/68993
dc.language.isoenen_ZA
dc.publisherAOSIS Open Journalsen_ZA
dc.rights© 2018. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.en_ZA
dc.subjectDiagnostic laboratoriesen_ZA
dc.subjectSequencingen_ZA
dc.subjectPolymerase chain reaction (PCR)en_ZA
dc.subjectDetectionen_ZA
dc.subjectJanus kinase 2 V617F mutationen_ZA
dc.subject.otherHealth sciences articles SDG-03
dc.subject.otherSDG-03: Good health and well-being
dc.subject.otherHealth sciences articles SDG-09
dc.subject.otherSDG-09: Industry, innovation and infrastructure
dc.subject.otherHealth sciences articles SDG-17
dc.subject.otherSDG-17: Partnerships for the goals
dc.titleDetection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assayen_ZA
dc.typeArticleen_ZA

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