Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
Loading...
Date
Authors
Senamela, Tshiphiri
Kock, Marleen M.
Becker, Piet J.
Potgieter, Joachim
Journal Title
Journal ISSN
Volume Title
Publisher
AOSIS Open Journals
Abstract
The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay
for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories.
Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used
to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and
PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens
harbouring the mutation.
Description
Keywords
Diagnostic laboratories, Sequencing, Polymerase chain reaction (PCR), Detection, Janus kinase 2 V617F mutation
Sustainable Development Goals
Citation
Senamela T, Kock M, Becker
P, et al. Detection of the
Janus kinase 2 V617F
mutation using a locked
nucleic-acid, real-time
polymerase chain reaction
assay. African Journal of Laboratory Medicine 2018;7(1), a658. https://DOI.org/10.4102/ajlm.v7i1.658.