Loop-mediated isothermal aplification detection of SARS-CoV-2 and myriad other applications
Moore, Keith J. M.; Cahill, Jeremy; Aidelberg, Guy; Aronoff, Rachel; Bektas, Ali; Bezdan, Daniela; Butler, Daniel J.; Chittur, Sridar V.; Codyre, Martin; Federici, Fernan; Tanner, Nathan A.; Tighe, Scott W.; True, Randy; Ware, Sarah B.; Wyllie, Anne L.; Afshin, Evan E.; Bendesky, Andres; Chang, Connie B.; Dela Rosa, Richard II; Elhaik, Eran; Erickson, David; Goldsborough, Andrew S.; Grills, George; Hadasch, Kathrin; Hayden, Andrew; Her, Seong-Young; Karl, Julie A.; Kim, Chang Hee; Kriegel, Alison J.; Kunstman, Thomas; Landau, Zeph; Land, Kevin; Langhorst, Bradley W.; Lindner, Ariel B.; Mayer, Benjamin E.; McLaughlin, Lee A.; McLaughlin, Matthew T.; Molloy, Jenny; Mozsary, Christopher; Nadler, Jerry L.; D’Silva, Melinee; Ng, David; O’Connor, David H.; Ongerth, Jerry E.; Osuolale, Olayinka; Pinharanda, Ana; Plenker, Dennis; Ranjan, Ravi; Rosbash, Michael; Rotem, Assaf; Segarra, Jacob; Schurer, Stephan; Sherrill-Mix, Scott; Solo-Gabriele, Helena; To, Shaina; Vogt, Merly C.; Yu, Albert D.; Mason, Christopher E.; The gLAMP Consortium
Date:
2022-09
Abstract:
As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test
for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future
outbreaks. The current gold standard for molecular diagnostics is the polymerase chain reaction (PCR), but the
extraordinary and unmet demand for testing in a variety of environments means that both complementary and
supplementary testing solutions are still needed. This review highlights the role that loop-mediated isothermal
amplification (LAMP) has had in filling this global testing need, providing a faster and easier means of testing, and
what it can do for future applications, pathogens, and the preparation for future outbreaks. This review describes the
current state of the art for research of LAMP-based SARS-CoV-2 testing, as well as its implications for other
pathogens and testing. The authors represent the global LAMP (gLAMP) Consortium, an international research
collective, which has regularly met to share their experiences on LAMP deployment and best practices; sections are
devoted to all aspects of LAMP testing, including preanalytic sample processing, target amplification, and amplicon
detection, then the hardware and software required for deployment are discussed, and finally, a summary of the
current regulatory landscape is provided. Included as well are a series of first-person accounts of LAMP method
development and deployment. The final discussion section provides the reader with a distillation of the most
validated testing methods and their paths to implementation. This review also aims to provide practical information
and insight for a range of audiences: for a research audience, to help accelerate research through sharing of best
practices; for an implementation audience, to help get testing up and running quickly; and for a public health,
clinical, and policy audience, to help convey the breadth of the effect that LAMP methods have to offer.
