dc.contributor.author |
Paradza, Vongai M.
|
|
dc.contributor.author |
Khamis, Fathiya M.
|
|
dc.contributor.author |
Yusuf, Abdullahi Ahmed
|
|
dc.contributor.author |
Subramanian, Sevgan
|
|
dc.contributor.author |
Akutse, Komivi S.
|
|
dc.date.accessioned |
2023-09-04T08:34:57Z |
|
dc.date.available |
2023-09-04T08:34:57Z |
|
dc.date.issued |
2022-09 |
|
dc.description |
DATA AVAILABILITY STATEMENT : The original contributions presented in the study are included in the article. Further inquiries can be directed to the corresponding author. |
en_US |
dc.description.abstract |
The efficiency of an autodissemination technique in controlling adult whiteflies, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae) on tomato, Solunum lycopersicum was investigated with previously identified potent fungal isolates of Metarhizium anisopliae ICIPE 18, ICIPE 62 and ICIPE 69 under screenhouse or semi-field conditions. The autodissemination device was inoculated with dry conidia of the M. anisopliae isolates, while control insects were exposed to a fungus–free device. Sampling for conidia uptake, conidial viability and persistence, and insect mortality was done at 1, 2, 3, 5 and 8 days post–exposure, and collected insects were monitored for mortality over ten days. Overall, mortality was higher in insects exposed to ICIPE 18 (62.8%) and ICIPE 69 (61.8%) than in those exposed to ICIPE 62 (42.6%), with median lethal times, (LT50) ranging between 6.73–8.54 days. The control group recorded the lowest mortality rates (18.9%). A general linear reduction in conidial viability with exposure time was observed, although this was more pronounced with M. anisopliae ICIPE 62. Insects exposed to M. anisopliae ICIPE 69 also recorded the highest conidia uptake, hence selected for further evaluation with a T. vaporariorum attractant volatile organic compound, (E)–2–hexenal. The volatile inhibited fungal germination in laboratory compatibility tests, therefore, spatial separation of M. anisopliae ICIPE 69 and (E)–2–hexenal in the autodissemination device was conducted. The inhibitory effects of the volatile were significantly reduced by spatial separation at a distance of 5 cm between the fungus and the volatile, which was found to be more suitable and chosen for the subsequent experiments. Results showed that (E)–2–hexenal did not influence conidia uptake by the insects, while fungal viability and the subsequent mortality variations were more related to duration of exposure. The fungus–volatile compatibility demonstrated with spatial separation provides a basis for the optimisation of the volatile formulation to achieve better T. vaporariorum suppression with an excellent autodissemination efficiency when used in the management of whiteflies under screenhouse conditions. |
en_US |
dc.description.department |
Forestry and Agricultural Biotechnology Institute (FABI) |
en_US |
dc.description.department |
Zoology and Entomology |
en_US |
dc.description.librarian |
hj2023 |
en_US |
dc.description.sponsorship |
BioInnovate Africa Phase I
project “Promoting smallholder access to fungal biopesticides
through Public Private Partnerships in East Africa” (BA/CI/
2017-02/PROSAFE), and UK’s Foreign, Commonwealth and
Development Office (FCDO) (FCDO Biopesticide Project,
B2291A - FCDO - BIOPESTICIDE) through the International
Centre of Insect Physiology and Ecology (icipe). The icipe core funding provided by UK’s Foreign,
Commonwealth and Development Office (FCDO); Swedish
International Development Cooperation Agency (Sida); the
Swiss Agency for Development and Cooperation (SDC); the
Federal Democratic Republic of Ethiopia; and the Government
of the Republic of Kenya. |
en_US |
dc.description.uri |
https://www.frontiersin.org/journals/insect-science |
en_US |
dc.identifier.citation |
Paradza, V.M., Khamis, F.M., Yusuf, A.A., Subramanian,, S., Akutse, K.S. (2022) Efficacy of Metarhizium anisopliae and (E)–2–hexenal combination using
autodissemination technology for the management of the adult greenhouse
whitefly, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae). Frontiers in Insect Science 2:991336. doi: 10.3389/finsc.2022.991336. |
en_US |
dc.identifier.issn |
2673-8600 (online) |
|
dc.identifier.other |
10.3389/finsc.2022.991336 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/92152 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Frontiers Media |
en_US |
dc.rights |
© 2022 Paradza, Khamis, Yusuf,
Subramanian and Akutse. This is an
open-access article distributed under
the terms of the Creative Commons
Attribution License (CC BY). |
en_US |
dc.subject |
Autodissemination device |
en_US |
dc.subject |
Entomopathogenic fungi |
en_US |
dc.subject |
Fungus–volatile compatibility |
en_US |
dc.subject |
Lure and infect |
en_US |
dc.subject |
Biopesticide |
en_US |
dc.subject |
SDG-15: Life on land |
en_US |
dc.subject |
Whitefly (Trialeurodes vaporariorum) |
en_US |
dc.title |
Efficacy of Metarhizium anisopliae and (E)–2–hexenal combination using autodissemination technology for the management of the adult greenhouse whitefly, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae |
en_US |
dc.type |
Article |
en_US |