Abstract:
Resistance to the antibiotic Cycloheximide has been reported for a number of fungal taxa. In particular, some yeasts are known to be highly resistant to this antibiotic. Early research showed that this resulted from a transition mutation in one of the 60S ribosomal protein genes. In addition to the yeasts, most genera and species in the Ophiostomatales are highly resistant to this antibiotic, which is widely used to selectively isolate these fungi. Whole-genome sequences are now available for numerous members of the Ophiostomatales providing an opportunity to determine whether the mechanism of resistance in these fungi is the same as that reported for yeast genera such as Kluyveromyces. We examined all the available genomes for the Ophiostomatales and discovered that a transition mutation in the gene coding for ribosomal protein eL42, which results in the substitution of the amino acid Proline to Glutamine, likely confers resistance to this antibiotic. This change across all genera in the Ophiostomatales suggests that the mutation arose early in the evolution of these fungi.
Description:
DATA AVAILABILTY : The genome data used in this study are available in the NCBI repository, https://www.ncbi.nlm.nih.gov. The accession numbers for all genomes are indicated in Table 1. The Microascus genome data is available on the JGI mycocosm website (https://mycocosm.jgi.doe.gov/mycocosm/home). The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
