The transmission of Babesia bovis using frozen infective material obtained from Boophilus microplus larvae

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The transmission of Babesia bovis using frozen infective material obtained from Boophilus microplus larvae

Van Vuuren, A.S.; Potgieter, F.T.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Verster, Anna J.M.; Walker, Jane B.
URI: http://hdl.handle.net/2263/54029
Date: 1974

Abstract:

Boophilus microplus larvae infected with Babesia bovis were removed from a susceptible ox approximately 72 hours after attachment, triturated and stored in liquid nitrogen refrigerator with dimethyl sulphoxide as cryoproctectant. When inoculated into splenectomised cattle the suspension was infective in the 2 animals into which it was injected intravenously but not in the 3rd, which was only injected subcutaneously. The prepatent period in the infected cattle was 10 and 11 days respectively.

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