Studies on schistosomiasis. 4. Differential staining of live and dead cercariae after immobilization with physostigmin

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Studies on schistosomiasis. 4. Differential staining of live and dead cercariae after immobilization with physostigmin

Van Wyk, Jan Aucamp; De Kock, V.E.; De Lange, M.; Bigalke, R.D.; Howell, P.G.; Reinecke, R.K.; Walker, Jane B.
URI: http://hdl.handle.net/2263/53674
Date: 1973

Abstract:

A modified eosin technique similar to that used for differential staining of live and dead spermatozoa stained dead cercariae of Schistosoma mattheei red while those that were alive remained colourless and transparent. Prior to exposure to the dye live cercariae were immobilized with physostigmin, which was better for that purpose than five other substances tested. The lowest concentration of physostigmin to immobilize cercariae quickly and effectively was found to be 3,3 x 10-⁶ M.

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