The isolation and identification of an antigen for the diagnosis of bovine mastitis by radial immunodiffusion

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The isolation and identification of an antigen for the diagnosis of bovine mastitis by radial immunodiffusion

Viljoen, M.H.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Verster, Anna J.M.; Walker, Jane B.
URI: http://hdl.handle.net/2263/53659
Date: 1974

Abstract:

An antigen associated with the somatic cells obtained from mastitic milk, which is also present in the whey and can be used for the diagnosis of mastitis in a radial immunodiffusion (RID) test, has been purified and identified. It is water soluble, with an approximate pI of 4,4 and S ₂₀w =4,35 S. The antigen was shown to be immunologically identical to bovine serum albumin by means of Ouchterlony and immunodiffusion techniques. The use of monospecific rabbit antiserum facilitates standardization of the test results

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