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Browsing by UP Author "Van Kleef, Mirinda"
Now showing items 1-17 of 17
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Faber, Erika; Tshilwane, Selaelo Ivy; Van Kleef, Mirinda; Pretorius, Alri (Elsevier, 2022-01)Expanding on our previous work, this study used transcriptome analysis of RNA sequences to investigate the various factors that contributed to either inducing apoptosis that resulted in cell death or promoting the survival ...
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Thema, Nontobeko; Pretorius, Alri; Tshilwane, Selaelo Ivy; Liebenberg, Junita; Steyn, Helena; Van Kleef, Mirinda (AOSIS OpenJournals, 2016-08-30)Secreted proteins are reported to induce cell-mediated immunity characterised by the production of interferon-gamma (IFN)-γ. In this study three open reading frames (ORFs) (Erum8060, Erum7760, Erum5000) encoding secreted ...
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Thema, Nontobeko; Tshilwane, Selaelo Ivy; Son, L.; Smith, R.M.; Faber, Frederika E.; Steyn, Helena C.; Van Kleef, Mirinda; Liebenberg, Junita; Pretorius, Alri (Elsevier, 2019-01)Since CD8+ T cells play an important role in resistance to infection with heartwater, effective vaccines against this disease will likely require identification of antigens that contain CD8+ T cell epitopes responsible for ...
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Thema, Nontobeko; Tshilwane, Selaelo Ivy; Pretorius, Alri; Son, L.; Smith, R.M.; Steyn, Helena C.; Liebenberg, Junita; Van Kleef, Mirinda (Elsevier, 2019-03)Several studies have shown that cytotoxic T lymphocytes (CTL) require CD4 + Th1 epitopes to generate strong immune responses to intracellular pathogens. However, not much is known about Ehrlichia ruminantium epitopes, ...
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Liebenberg, Junita; Faber, F.E.; Collins, Nicola E.; Allsopp, Basil A.; Van Kleef, Mirinda (Elsevier, 2012-01)Ehrlichia ruminantium is an obligate intracellular bacterial pathogen which causes heartwater, a serious tick-borne disease of ruminants throughout sub-Saharan Africa. The development of promising recombinant vaccines ...
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Faber, Erika; Van Schalkwyk, Antoinette; Tshilwane, Selaelo Ivy; Van Kleef, Mirinda; Pretorius, Alri (Elsevier, 2024-01)The viral proteins VP1-1, VP2, VP4, VP7 and NS3, of African horse sickness virus serotype 4 (AHSV4), have previously been identified to contain CD8+ T cell epitopes. In this study, overlapping peptides spanning the entire ...
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Van Kleef, Mirinda; Jardine, J.E.; Vogel, S.W.; Van der Lugt, Jaco J.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)An immunohistochemical staining technique in which a monospecific serum was used against the major antigenic protein-1 (MAP-1) of Cowdria ruminantium, was evaluated for the detection of C. ruminantium in formalin-fixed ...
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Faber, Erika; Tshilwane, Selaelo Ivy; Van Kleef, Mirinda; Pretorius, Alri (Elsevier, 2022-12)Transcriptome analysis was used to characterise the in vitro primary and secondary immune responses induced in horse peripheral blood mononuclear cells (PBMC) stimulated for 24 h with the individual recombinant proteins ...
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Sebatjane, Selaelo Ivy; Pretorius, Alri; Liebenberg, Junita; Steyn, H.; Van Kleef, Mirinda (Elsevier, 2010-10)Low molecular weight (LMW) proteins of E. ruminantium can induce proliferation of immune peripheral blood mononuclear cells (PBMCs) and the production of interferongamma (IFN-y ) by CD4+-enriched T-cells. In this study, ...
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Tshilwane, Selaelo Ivy; Thema, Nontobeko; Steyn, Helena C.; Van Kleef, Mirinda; Pretorius, Alri (Elsevier, 2019-07)Previously, a heartwater experimental DNA vaccine provided 100% protection following laboratory challenge with Ehrlichia ruminantium administered by needle but not against an E. ruminantium tick challenge in the field. A ...
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Tshikhudoa, Ndavheleseni; Pretorius, Alri; Putterill, John Fraser; Van Kleef, Mirinda (Elsevier, 2010-08)Four E. ruminantium 1H12 open reading frames and their proteins known to protect sheep against heartwater needle challenge were encapsulated into, or adsorbed onto poly (d,l-lactide-co-glycolide) microparticles. Microspheres ...
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Pretorius, Alri; Collins, Nicola E.; Steyn, H.C.; Van Strijp, M.F.; Van Kleef, Mirinda; Allsopp, Basil A. (Elsevier, 2007-03-08)We have reported previously that a recombinant DNA vaccine consisting of four Ehrlichia ruminantium (Welgevonden) open reading frames (ORFs) known as the 1H12 cocktail provided protection against a virulent E. ruminantium ...
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Steyn, H.C.; Pretorius, Alri; McCrindle, Cheryl Myra Ethelwyn; Steinmann, C.M.L.; Van Kleef, Mirinda (Elsevier, 2008)Heartwater is a tick borne disease that affects ruminants and wild animals in Africa south of the Sahara. It is caused by Ehrlichia ruminantium and transmitted by the tick Amblyomma hebraeum. The protocols currently used ...
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Pretorius, Alri; Nefefe, T.; Thema, N.; Liebenberg, J.; Steyn, H.; Van Kleef, Mirinda (Elsevier, 2024-04)Heartwater is one of the most economically important tick-borne fatal diseases of livestock. The disease is caused by the bacteria Ehrlichia ruminantium transmitted by Amblyomma ticks. Although there is evidence that ...
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Ngoepe, Tlou A.; Pretorius, Alri; Steyn, Helena C.; Van Kleef, Mirinda (AOSIS, 2023-03-23)Cowdria polymorphic gene 1 (cpg1, Erum2510, ERUM_RS01380) has been shown to induce 30% and 100% protection in sheep immunised by deoxyribonucleic acid (DNA) prime combined with DNA boost and DNA prime combined with protein ...
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Tjale, Mabotse A.; Pretorius, Alri; Josemans, Antoinette; Van Kleef, Mirinda; Liebenberg, Junita (Elsevier, 2018-01)The use of bioinformatics tools to search for possible vaccine candidates has been successful in recent years. In an attempt to search for additional vaccine candidates or improve the current heartwater vaccine design, a ...
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Faber, Erika; Tshilwane, Selaelo Ivy; Van Kleef, Mirinda; Pretorius, Alri (Elsevier, 2021-07)African horse sickness (AHS) is caused by African horse sickness virus (AHSV), a double stranded RNA (dsRNA) virus of the genus Orbivirus, family Reoviridae. For the development of new generation AHS vaccines or antiviral ...
Now showing items 1-17 of 17