Evaluation of microsatellites for common ungulates in the South African wildlife industry

dc.contributor.authorMiller, Susan M.
dc.contributor.authorClarke, Amy B.
dc.contributor.authorBloomer, Paulette
dc.contributor.authorGuthrie, Alan John
dc.contributor.authorHarper, Cindy Kim
dc.date.accessioned2016-08-16T09:53:50Z
dc.date.issued2016-09
dc.description.abstractWildlife is a multi-million dollar industry in South Africa ranging from intensively farmed animals on small properties to free-roaming animals in large functional ecosystems. Specific concerns surrounding the conservation value of the intensively managed wildlife have been raised. Increasingly it is recognised that genetics must be considered when developing conservation policy and management practices. Minimal genetic data are available to assist with decisions within the industry. Microsatellite markers have been developed for some species and are currently used for individual identification, parentage assessment and to inform translocation decisions. However, validation for standardized application in wildlife management has not been completed. Common species for which conservation management decisions have to be made include: Cape buffalo (Syncerus caffer), blue wildebeest (Connochaetes taurinus taurinus), common impala (Aepyceros melampus) and sable antelope (Hippotragus niger). Using existing microsatellite markers for domestic and wild species, we have developed multiplex panels for use in these species. We discriminated between 751 buffalo (four pairs were not distinguishable), 1307 blue wildebeest (three pairs were not distinguishable), 580 impala and 493 sable antelope. We determined parentage in four case studies using a combination of microsatellite and demographic data. We also tested microsatellite markers on roan antelope (Hippotragus equinus), gemsbok (Oryx gazella) and eland (Tragelaphus oryx) and report preliminary data. Further research to improve this validation includes: extensive sampling from more diverse areas, targeted or full genome sequencing to identify additional microsatellite loci, development of sampling kits and training of veterinarians, as well as expansion of the species tested.en_ZA
dc.description.departmentEquine Research Centreen_ZA
dc.description.departmentGeneticsen_ZA
dc.description.embargo2017-09-30
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipSMM was partially funded by a Post-doctoralFellowship from the Faculty of Natural and Agricultural Sciences of the University of Pretoria.en_ZA
dc.description.urihttp://link.springer.com/journal/12686en_ZA
dc.identifier.citationMiller, S.M., Clarke, A.B., Bloomer, P., Guthrie, A.J. & Harper, C.K. Evaluation of microsatellites for common ungulates in the South African wildlife industry. Conservation Genetics Resources (2016) 8: 329-341. doi:10.1007/s12686-016-0554-7.en_ZA
dc.identifier.issn1877-7252 (print)
dc.identifier.issn1877-7260 (online)
dc.identifier.other10.1007/s12686-016-0554-7
dc.identifier.urihttp://hdl.handle.net/2263/56352
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Springer Science+Business Media Dordrecht 2016. The original publication is available at : http://link.springer.comjournal/12686.en_ZA
dc.subjectConservation geneticsen_ZA
dc.subjectMicrosatellite markersen_ZA
dc.subjectUngulatesen_ZA
dc.subjectStandardisationen_ZA
dc.subjectSouth Africa (SA)en_ZA
dc.titleEvaluation of microsatellites for common ungulates in the South African wildlife industryen_ZA
dc.typePostprint Articleen_ZA

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