Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney disease

dc.contributor.authorChandler, Jennifer C.
dc.contributor.authorJafree, Daniyal J.
dc.contributor.authorMalik, Saif
dc.contributor.authorPomeranz, Gideon
dc.contributor.authorBall, Mary
dc.contributor.authorKolatsi-Joannou, Maria
dc.contributor.authorPiapi, Alice
dc.contributor.authorMason, William J.
dc.contributor.authorBenest, Andrew V.
dc.contributor.authorBates, David O.
dc.contributor.authorLetunovska, Aleksandra
dc.contributor.authorAl-Saadi, Reem
dc.contributor.authorRabant, Marion
dc.contributor.authorBoyer, Olivia
dc.contributor.authorPritchard-Jones, Kathy
dc.contributor.authorWinyard, Paul J.
dc.contributor.authorMason, Andrew S.
dc.contributor.authorWoolf, Adrian S.
dc.contributor.authorWaters, Aoife M.
dc.contributor.authorLong, David A.
dc.date.accessioned2024-10-31T07:28:20Z
dc.date.available2024-10-31T07:28:20Z
dc.date.issued2024-10
dc.descriptionDATA AVAILABILITY STATEMENT : Primary sequencing data reported in this paper are available on NCBI Sequence Read Archive (SRA PRJNA928337). Processed data for scRNA-seq experiments are available on Zenodo at DOI: 10.5281/zenodo.7565867. The codes generated during this study are available at Github repository https://github.com/daniyal-jafree1995/collaborations. All other unique reagents used in this study are available from the corresponding author on reasonable request.en_US
dc.descriptionSUPPLEMENTARY MATERIALS AND METHODS : FIGURE S1. Supporting data for single-cell RNA seq analyses. FIGURE S2. Characterisation of primary cell lines. FIGURE S3. Isolation of podocytes from scRNA-seq data from additional murine models of glomerular disease for cross-disease comparison. FIGURE S4. Dysregulated genes shown by individual sample for each human glomerular pathology (source Nephroseq). TABLE S1. Differentially expressed genes in glomerular cells in Wt1R394W/+ mice. TABLE S2. Podocyte differentially expressed genes that are conserved across murine glomerular scRNA-seq datasets or unique to Wt1R394W/+. TABLE S3. Podocyte differentially expressed genes classified in ‘GO:0002376 – immune system process’ across murine glomerular scRNA-seq datasets.en_US
dc.description.abstractPlease read abstract in the article.en_US
dc.description.departmentMedical Oncologyen_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-03:Good heatlh and well-beingen_US
dc.description.sponsorshipLifeArc/Great Ormond Street Children's Charity; Medical Research Centre; Kidney Research UK; British Heart Foundation; Diabetes UK; Wellcome Trust.en_US
dc.description.urihttps://onlinelibrary.wiley.com/journal/10969896en_US
dc.identifier.citationChandler J.C., Jafree D.J., Malik S. et al. 2024, 'Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney disease', Journal of Pathology, vol. 264, no. 2, pp. 212-227, doi : 10.1002/path.6339.en_US
dc.identifier.issn0022-3417 (print)
dc.identifier.issn1096-9896 (online)
dc.identifier.other10.1002/path.6339
dc.identifier.urihttp://hdl.handle.net/2263/98857
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. This is an open access article under the terms of the Creative Commons Attribution License.en_US
dc.subjectVascular endothelial growth factor-Aen_US
dc.subjectWT1 glomerulopathyen_US
dc.subjectWilms tumour 1 (WT1)en_US
dc.subjectSingle-cell transcriptomicsen_US
dc.subjectPodocyteen_US
dc.subjectNeuropilin-1en_US
dc.subjectGlomerular endotheliumen_US
dc.subjectChildhood glomerular diseaseen_US
dc.subjectAdrenomedullinen_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.titleSingle-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney diseaseen_US
dc.typeArticleen_US

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