Acute animal toxicity and genotoxicity of obliquumol, a potential new framework antifungal compound isolated from Ptaeroxylon obliquum (Rutaceae) leaf extracts

dc.contributor.authorRamadwa, Thanyani Emelton
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorMadikizela, Balungile
dc.contributor.authorEloff, Jacobus Nicolaas
dc.date.accessioned2025-05-15T11:53:25Z
dc.date.issued2024-09
dc.description.abstractObliquumol (12-O-acetylptaeroxylinol) isolated from Ptaeroxylon obliquum leaves has excellent antifungal activity and low cellular toxicity. As a next step in the potential development of a framework antifungal product, the present work investigated the acute animal toxicity of obliquumol according to OECD 423 guidelines. Furthermore, the genotoxicity of P. obliquum acetone leaf extracts, fractions (hexane, chloroform and 30 % H2O in MeOH) and isolated compounds (obliquumol and a mixture of lupeol and β-amyrin) was determined using Ames test. . A single dose of obliquumol was orally administered to mice at levels of 50, 300 and 2000 mg/kg and observed for 14 days. The three S. typhimurium tester strains TA 98, TA 100 and TA 102 were used without metabolic activation to determine the genotoxicity. Even at the highest dose of obliquumol, the mass, behaviour and food intake of the animals were not affected. Gross necropsy and histopathological analysis on organs indicated hardly any effects. No samples had genotoxic activity against the S. typhimurium strains tested. Obliquumol had an LD50 >2000 mg/kg since there were no mortalities after 14 days. This encourages the possible development of a new class of antifungal compounds from the obliquumol framework.
dc.description.departmentParaclinical Sciences
dc.description.embargo2025-07-18
dc.description.librarianhj2025
dc.description.sdgSDG-03: Good health and well-being
dc.description.sdgSDG-15: Life on land
dc.description.sponsorshipThe National Research Foundation and Technology and Innovation Agency (TIA).
dc.description.urihttp://www.elsevier.com/locate/sajb
dc.identifier.citationRamadwa, T.E., McGaw, L.J., Madikizela, B. & Eloff, J.N. 2024, 'Acute animal toxicity and genotoxicity of obliquumol, a potential new framework antifungal compound isolated from Ptaeroxylon obliquum (Rutaceae) leaf extracts', South African Journal of Botany, vol. 172, pp. 175-179, doi : 10.1016/j.sajb.2024.07.025.
dc.identifier.issn0254-6299 (print)
dc.identifier.issn1727-9321 (online)
dc.identifier.other10.1016/j.sajb.2024.07.025
dc.identifier.urihttp://hdl.handle.net/2263/102405
dc.language.isoen
dc.publisherElsevier
dc.rights© 2024 Published by Elsevier B.V. on behalf of SAAB. Notice : this is the author’s version of a work that was accepted for publication in South African Journal of Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in South African Journal of Botany, vol. 172, pp. 175-179, 2024, doi : 10.1016/j.sajb.2024.07.025.
dc.subjectPtaeroxylon obliquum
dc.subjectObliquumol
dc.subjectAcute toxicity
dc.subjectGenotoxicity
dc.subjectAmes test
dc.titleAcute animal toxicity and genotoxicity of obliquumol, a potential new framework antifungal compound isolated from Ptaeroxylon obliquum (Rutaceae) leaf extracts
dc.typePostprint Article

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