Sapwood mycobiome varies across host, plant compartment and environments in Nothofagus forests from Northern Patagonia

dc.contributor.authorMolina, Lucia
dc.contributor.authorRajchenberg, Mario
dc.contributor.authorDe Errasti, Andres
dc.contributor.authorVogel, Braian
dc.contributor.authorCoetzee, Martin Petrus Albertus
dc.contributor.authorAime, Mary Catherine
dc.contributor.authorPildain, Maria Belen
dc.date.accessioned2023-02-08T06:03:08Z
dc.date.issued2023-12
dc.descriptionDATA AVAILABILITY STATEMENT : Raw sequence reads are deposited in the Short Read Archive of the National Center for Biotechnology Information (BioProject ID: PRJNA785007).en_US
dc.description.abstractGlobal forests are increasingly being threatened by altered climatic conditions and increased attacks by pests and pathogens. The complex ecological interactions among pathogens, microbial communities, tree hosts and the environment are important drivers of forest dynamics. Little is known about the ecology of forest pathology and related microbial communities in temperate forests of the southern hemisphere. In this study, we used next-generation sequencing to characterize sapwood-inhabiting fungal communities in North Patagonian Nothofagus forests and assessed patterns of diversity of taxa and ecological guilds across climatic, site and host variables (health condition and compartment) as a contribution to Nothofagus autecology. The diversity patterns inferred through the metabarcoding analysis were similar to those obtained through culture-dependent approaches. However, we detected additional heterogeneity and greater richness with culture-free methods. Host species was the strongest driver of fungal community structure and composition, while host health status was the weakest. The relative impacts of site, season, plant compartment and health status were different for each tree species; these differences can be interpreted as a matter of water availability. For Nothofagus dombeyi, which is distributed across a wide range of climatic conditions, site was the strongest driver of community composition. The microbiome of N. pumilio varied more with season and temperature, a relevant factor for forest conservation in the present climate change scenario. Both species carry a number of potential fungal pathogens in their sapwood, whether they exhibit symptoms or not. Our results provide insight into the diversity of fungi associated with the complex pathobiome of the dominant Nothofagus species in southern South America.en_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.departmentGeneticsen_US
dc.description.embargo2023-11-07
dc.description.librarianhj2023en_US
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas and Fondo para la Investigación Científica y Tecnológica.en_US
dc.description.urihttp://www.wileyonlinelibrary.com/journal/mecen_US
dc.identifier.citationMolina, L., Rajchenberg, M., de Errasti, A., Vogel, B., Coetzee, M. P. A., Aime, M. C., & Pildain, M. B. (2023) Sapwood mycobiome varies across host, plant compartment and environments in Nothofagus forests from Northern Patagonia. Molecular Ecology vol. 32, no. 23, pp. 6599-6618. https://doi.org/10.1111/mec.16771.en_US
dc.identifier.issn0962-1083 (print)
dc.identifier.issn1365-294X (online)
dc.identifier.other10.1111/mec.16771
dc.identifier.urihttps://repository.up.ac.za/handle/2263/89271
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2022 John Wiley & Sons Ltd. This is the pre-peer reviewed version of the following article : Sapwood mycobiome varies across host, plant compartment and environments in Nothofagus forests from Northern Patagonia. Molecular Ecology, vol. 32, no. 23, pp. 6599-6618, 2023, https://doi.org/10.1111/mec.16771. The definite version is available at : http://wileyonlinelibrary.com/journal/mec.en_US
dc.subjectEnvironmental DNAen_US
dc.subjectDeoxyribonucleic acid (DNA)en_US
dc.subjectForest declineen_US
dc.subjectLatent pathogensen_US
dc.subjectMetabarcodingen_US
dc.subjectTemperate forestsen_US
dc.subjectWood endophyteen_US
dc.titleSapwood mycobiome varies across host, plant compartment and environments in Nothofagus forests from Northern Patagoniaen_US
dc.typePostprint Articleen_US

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