Direct culture–independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses
| dc.contributor.author | May, Catherine Edith | |
| dc.contributor.author | Guthrie, Alan John | |
| dc.contributor.author | Schulman, M.L. (Martin) | |
| dc.contributor.email | kate.may@up.ac.za | en_ZA |
| dc.date.accessioned | 2020-02-28T12:21:20Z | |
| dc.date.available | 2020-02-28T12:21:20Z | |
| dc.date.issued | 2019-09 | |
| dc.description.abstract | We report herein the use of crude extracts obtained from samples of Taylorella equigenitalis–infected horses for the purpose of multi-locus sequence typing (MLST). Samples (n = 36) were collected from horses in South Africa from 1996 to 2017: 34 from genital swabs (stored at −20°C for 2–3 y) and 2 from cryopreserved raw semen aliquots (stored at −70°C for 18 y) prior to assay. The MLST assay showed a single sequence type (ST), designated ST4, that supported a point introduction and thus a common source for the South African outbreak of contagious equine metritis. | en_ZA |
| dc.description.department | Equine Research Centre | en_ZA |
| dc.description.department | Production Animal Studies | en_ZA |
| dc.description.librarian | hj2020 | en_ZA |
| dc.description.uri | https://journals.sagepub.com/home/vdi | en_ZA |
| dc.identifier.citation | May, C.E., Guthrie, A.J. & Schulman, M.L. 2019, 'Direct culture–independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses', Journal of Veterinary Diagnostic Investigation, vol. 31, no. 5, pp. 792-794. | en_ZA |
| dc.identifier.issn | 1040-6387 (print) | |
| dc.identifier.issn | 1943-4936 (online) | |
| dc.identifier.other | 10.1177/1040638719871089 | |
| dc.identifier.uri | http://hdl.handle.net/2263/73609 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | Sage | en_ZA |
| dc.rights | © 2019 The Author(s) | en_ZA |
| dc.subject | Contagious equine metritis (CEM) | en_ZA |
| dc.subject | Direct culture–independent strain typing | en_ZA |
| dc.subject | Taylorella equigenitalis | en_ZA |
| dc.subject | Horse (Equus caballus) | en_ZA |
| dc.subject | South Africa (SA) | en_ZA |
| dc.title | Direct culture–independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses | en_ZA |
| dc.type | Postprint Article | en_ZA |