Phylogeny of multiple genomic regions of infectious laryngotracheitis virus in Turkish poultry flocks

dc.contributor.authorAydin, O.
dc.contributor.authorBayraktar, E.
dc.contributor.authorTali, H.E.
dc.contributor.authorOzkan, I.E.
dc.contributor.authorYilmaz, A.
dc.contributor.authorUmar, S.
dc.contributor.authorBamac, O.E.
dc.contributor.authorTuran, N.
dc.contributor.authorKonuk, C.
dc.contributor.authorSadeyen, J.-R.
dc.contributor.authorChang, P.
dc.contributor.authorRicht, Juergen A.
dc.contributor.authorIqbal, M.
dc.contributor.authorYilmaz, H.
dc.date.accessioned2025-05-20T05:13:22Z
dc.date.available2025-05-20T05:13:22Z
dc.date.issued2025-05
dc.descriptionDATA AVAILABILITY STATEMENT : All data are included in the manuscript. The data is available up on request from the corresponding author. Sequences submitted to GenBank can be downloaded from NCBI (https://www.ncbi.nlm.nih.gov/nuccore).
dc.description.abstractInfectious laryngotracheitis (ILT) is an economically significant respiratory tract viral disease affecting poultry worldwide. There is a scarcity of data on the types of ILTV strains circulating in Turkey. This study aimed to determine the frequency and genotypic variations of Turkish ILTV strains. Commercial layer flocks (n = 14) and broiler flocks (n = 105) with a history of respiratory diseases were visited. From each flock, 5 to 10 birds from different age groups were necropsied. Clinical and pathological lesions were recorded, and tracheal tissue samples were collected for further studies. Nucleic acid was extracted from samples and subjected to ILTV detection using PCR assays. Clinical signs of anorexia, lethargy, swollen eyelids, mild to severe conjunctivitis, mucoid to purulent nasal discharge, and a drop in egg production were generally observed among ILTV-infected flocks. Pathological lesions, including conjunctivitis, mucoid to purulent sinusitis, and hemorrhagic tracheitis, were observed during necropsy. Among 119 flocks (14 layers and 105 broiler) analyzed in this study, 17 (17/119, 14.28 %) flocks were found positive for ILTV infection by PCR. Of the 17 ILTV-positive samples, 15 could be sequenced successfully for partial gB, gG, and ICP4 genes. Comparative analysis of partial ICP4 gene nucleotides revealed a unique 18 bp insertion "GCGGTTCTTGCGGTTGTT" among ILTV strains. Two nucleotide substitutions were observed in gB gene sequences at positions 5 (T to C) and 488 (A to G), resulting in amino acid substitutions at positions 2 (I to T) and 163 (K to R). Phylogenetic analysis of the gB gene revealed a close clustering (Cluster I) between ILTV strains from this study and those reported from China, Australia, and the USA. Phylogenetic analysis of gG gene sequences showed a close relation to ILTV strains from Russia, China, Canada, the USA, and Italy. No recombination events were observed among the partial sequences of ILTV genes analyzed in this study. Findings of this study show that ILTV infections are frequent in Turkish poultry flocks and contribute to our understanding of the genomic variations in gB, gG and ICP4 genes of ILTV which might help to mitigate ILTV infections in Turkey.
dc.description.departmentVeterinary Tropical Diseases
dc.description.librarianhj2025
dc.description.sdgSDG-03: Good health and well-being
dc.description.sdgSDG-02: Zero Hunger
dc.description.sponsorshipThe Istanbul University-Cerrahpasa-Teknokent; partially supported by the MCB core of the Center on Emerging and Zoonotic Infectious Diseases (CEZID) of the National Institute of General Medical Sciences (NIGMS) and by the Vanier-Krause BRI Endowed Professorship in Animal Infectious Diseases (JAR).
dc.description.urihttps://www.elsevier.com/locate/psj
dc.identifier.citationAydin, O., Bayraktar, E., Tali, H.E. et al. 2025, 'Phylogeny of multiple genomic regions of infectious laryngotracheitis virus in Turkish poultry flocks', Poultry Science, vol. 104, no. 5, art. 104957, pp. 1-10, doi : 10.1016/j.psj.2025.104957.
dc.identifier.issn0032-5791 (print)
dc.identifier.issn1525-3171 (online)
dc.identifier.other10.1016/j.psj.2025.104957
dc.identifier.urihttp://hdl.handle.net/2263/102432
dc.language.isoen
dc.publisherElsevier
dc.rights© 2025 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
dc.subjectInfectious laryngotracheitis (ILT)
dc.subjectInfectious laryngotracheitis virus (ILTV)
dc.subjectgB gene
dc.subjectgG gene
dc.subjectICP4 gene
dc.subjectPhylogeny
dc.subjectPoultry
dc.subjectRespiratory tract viral disease
dc.subjectTurkey
dc.titlePhylogeny of multiple genomic regions of infectious laryngotracheitis virus in Turkish poultry flocks
dc.typeArticle

Files

Original bundle

Now showing 1 - 5 of 8
Loading...
Thumbnail Image
Name:
Aydin_Phylogeny_2025.pdf
Size:
7.28 MB
Format:
Adobe Portable Document Format
Description:
Article
Loading...
Thumbnail Image
Name:
Aydin_PhylogenySuppl1_2025.docx
Size:
397.26 KB
Format:
Microsoft Word XML
Description:
Supplementary Material 1
Loading...
Thumbnail Image
Name:
Aydin_PhylogenySuppl2_2025.docx
Size:
261.4 KB
Format:
Microsoft Word XML
Description:
Supplementary Material 2
Loading...
Thumbnail Image
Name:
Aydin_PhylogenySuppl3_2025.docx
Size:
415.02 KB
Format:
Microsoft Word XML
Description:
Supplementary Material 3
Loading...
Thumbnail Image
Name:
Aydin_PhylogenySuppl4_2025.docx
Size:
437.09 KB
Format:
Microsoft Word XML
Description:
Supplementary Material 4

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: