Prevalence of antimalaria drug resistance-conferring mutations associated with sulphadoxine-pyrimethamineine-resistant Plasmodium falciparum in East Africa : a systematic review and meta-analysis

dc.contributor.authorAbebe, Wagaw
dc.contributor.authorAshagre, Agenagnew
dc.contributor.authorMisganaw, Tadesse
dc.contributor.authorDejazmach, Zelalem
dc.contributor.authorKumie, Getinet
dc.contributor.authorNigatie, Marye
dc.contributor.authorJemal, Abdu
dc.contributor.authorAsmare, Zelalem
dc.contributor.authorKassahun, Woldeteklehaymanot
dc.contributor.authorGedfie, Solomon
dc.contributor.authorGetachew, Ermias
dc.contributor.authorGashaw, Muluken
dc.contributor.authorAyana, Sisay
dc.contributor.authorGashaw, Yalewayker
dc.contributor.authorSisay, Assefa
dc.contributor.authorTadesse, Selamyhun
dc.contributor.authorEshetu, Tegegne
dc.contributor.authorAwoke, Mulat
dc.contributor.authorKassanew, Birhanu
dc.contributor.authorKidie, Atitegeb Abera
dc.contributor.authorAbate, Biruk Beletew
dc.contributor.authorReta, Melese Abate
dc.date.accessioned2025-05-28T07:39:33Z
dc.date.available2025-05-28T07:39:33Z
dc.date.issued2025-04
dc.descriptionAVAILABILITY OF DATA AND MATERIALS : No datasets were generated or analysed during the current study.
dc.description.abstractBACKGROUND : The emergence and spread of drug resistance to antimalarial drugs pose a severe threat to effective malaria control and treatment. Although sulfadoxine-pyrimethamine resistance is well-documented, it is still the drug of choice for treating intermittent resistance. Molecular markers play a crucial role in tracking and understanding the prevalence of antimalarial drug resistance. Currently, there is insufficient information on the prevalence of molecular markers associated with sulfadoxine-pyrimethamine resistance in P. falciparum. OBJECTIVE : This systematic review and meta-analysis aimed to determine the pooled prevalence of antimalaria drug resistance-conferring markers associated with sulphadoxine-pyrimethamineine in Plasmodium falciparum in East Africa. METHODS : Systematic searches was performed to retrieve articles from PubMed, Scopus, Science Direct databases, and Google Scholar search engine. Sixteen potential studies that provided important data on markers for sulphadoxine-pyrimethamineine resistance in Plasmodium falciparum were systematically reviewed and analyzed. Nine antimalarial drug resistance markers responsible for sulphadoxine-pyrimethamineine resistance in Plasmodium falciparum were extracted separately into Microsoft Excel and analyzed using STATA 17.0. The inverse of variance was done to evaluate heterogeneity across studies. A funnel plot was used to determine the presence of publication bias. A trim-and-fill-meta-analysis was carried out to generate a bias-adjusted effect estimate. A random effect model was used to determine the pooled prevalence of markers responsible for sulphadoxine-pyrimethamineine resistance. Subgroup analysis was performed based on country and year of publication. RESULTS : A total of 16 studies were included for this systematic review and meta-analysis.The molecular markers like dhfr (N51I, C59R, S108N, 108N, 59R, and I164L), and dhps (A437G, K540E, & 540E) were selected for meta-analysis. From this meta-analysis, the pooled prevalence of dhfr N51I, dhfr C59R, dhfr S108N, dhfr 108N, dhfr 59R, and dhfr I164L was 88.6%, 85.3%, 89.6%, 92.2%, 71.5%, and 3.9%, respectively. Likewise, the aggregated prevalence of dhps A437G, dhps K540E, and dhps 540E was 90.2%, 80.9%, and 91.5%, respectively. The subgroup analysis based on year of publication showed that the pooled prevalence of dhfr N51I, dhfr C59R, dhfr S108N, dhps A437G, and dhps K540E, in studies conducted 2014–2018 was 97.11%, 90.57%, 96.45%, 90.89%, and 89.45%, respectively, while it was 82.03%, 81.78%, 85.12%, 89.24%, and 73.98%, respectively, in studies conducted 2019–2023. On the other hand, country-based analysis showed that the pooled prevalence of dhfr N51I, dhfr C59R, dhfr S108N, dhps A437G, and dhps K540E, in Kenya was 85.88%, 84.02%, 86.56%, 90.7%, and 77.55%, respectively. CONCLUSIONS : This systematic review and meta-analysis reveal a high prevalence of drug resistance markers associated with sulphadoxine-pyrimethamine resistance in Plasmodium falciparum across the East African region. This underscores the significant challenges in managing malaria infections caused by Plasmodium falciparum in the region. Therefore, regular monitoring, identification, and limiting of drug-resistance markers and drug-resistant P. falciparum strains must be sustained to ensure the effectiveness of malaria treatment.
dc.description.departmentMedical Microbiology
dc.description.librarianhj2025
dc.description.sdgSDG-03: Good health and well-being
dc.description.urihttps://ann-clinmicrob.biomedcentral.com/
dc.identifier.citationAbebe, W., Ashagre, A., Misganaw, T. et al. Prevalence of antimalaria drug resistance-conferring mutations associated with sulphadoxine-pyrimethamineine-resistant Plasmodium falciparum in East Africa: a systematic review and meta-analysis. Annals of Clinical Microbiology and Antimicrobials 24, 25 (2025). https://doi.org/10.1186/s12941-025-00795-7.
dc.identifier.issn1476-0711 (online)
dc.identifier.other10.1186/s12941-025-00795-7
dc.identifier.urihttp://hdl.handle.net/2263/102551
dc.language.isoen
dc.publisherBioMed Central
dc.rights© The Author(s) 2025. Open Access, This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
dc.subjectPrevalence
dc.subjectPlasmodium falciparum
dc.subjectDrug resistance
dc.subjectMarker
dc.subjectSulphadoxine-pyrimethamineine
dc.subjectEast Africa
dc.titlePrevalence of antimalaria drug resistance-conferring mutations associated with sulphadoxine-pyrimethamineine-resistant Plasmodium falciparum in East Africa : a systematic review and meta-analysis
dc.typeArticle

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