In vitro effects of L-kynurenine and quinolinic acid on adhesion, migration and apoptosis in B16 F10 melanoma cells
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Date
Authors
Basson, Charlise
Serem, June Cheptoo
Bipath, Priyesh
Hlophe, Y.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
INTRODUCTION :
The inhibition of melanoma adhesion through adhesion molecules, such as integrins and E-cadherin, may represent a promising strategy for managing melanoma metastasis. Compounds, namely l-kynurenine (L-kyn) and quinolinic acid (Quin), previously displayed anti-cancer effects at half-maximal inhibitory concentration (IC50) against B16 F10 melanoma cells in vitro. However, the role of these compounds in B16 F10 melanoma cell adhesion, migration and apoptosis remain unknown.
METHODS :
Post-exposure to the compounds, flow cytometry was used to analyse the expression of very late antigen-5 (VLA-5), E-cadherin and cleaved caspase-3 in B16 F10 melanoma and RAW 264.7 murine macrophage cells. An adhesion assay was used to quantify the adhesion of both cell lines to vitronectin. A scratch migration assay was used to measure the possible inhibition of cell migration in B16 F10 cells in response to L-kyn and Quin.
RESULTS :
In both B16 F10 and RAW 264.7 cells, neither L-kyn nor Quin induced significant effects on VLA-5 expression or cell adhesion to vitronectin. In B16 F10 cells, both L-kyn and Quin elevated E-cadherin expression and displayed a trend of suppressed migration. However, only L-kyn elevated E-cadherin in RAW 264.7 cells. L-kyn induced apoptosis by elevating cleaved caspase-3 expression in both cell lines.
CONCLUSION :
L-kyn and Quin demonstrated promising antimetastatic effects in their ability to elevate E-cadherin expression and induce apoptosis in B16 F10 melanoma cells. However, these effects did not occur in response to vitronectin or VLA-5 integrin alterations. Furthermore, it cannot be excluded that L-kyn also induced apoptosis in RAW 264.7 cells. As such, these effects should be confirmed in additional control cell lines and substantiated with in vivo models.
Description
Keywords
Melanoma, Kynurenine metabolites, l-kynurenine, Quinolinic acid, Adhesion, Apoptosis, SDG-03: Good health and well-being
Sustainable Development Goals
SDG-03:Good heatlh and well-being
Citation
Basson, C., Serem, J.C., Bipath, P. & Hlophe, Y.N. 2024, 'In vitro effects of L-kynurenine and quinolinic acid on adhesion, migration and apoptosis in B16 F10 melanoma cells', Biochemical and Biophysical Research Communications, vol. 736, art. 150851, pp. 1-7, doi : 10.1016/j.bbrc.2024.150851.