Resistance of developing honeybee larvae during chronic exposure to dietary nicotine

dc.contributor.emailArcher@demogr.mpg.deen_ZA
dc.contributor.upauthorHuman, Hannelie
dc.contributor.upauthorArcher, C. Ruth
dc.contributor.upauthorDu Rand, Esther Elizabeth
dc.contributor.upauthorPirk, Christian Walter Werner
dc.contributor.upauthorNicolson, Sue W.
dc.date.accessioned2015-08-28T07:49:27Z
dc.date.available2015-08-28T07:49:27Z
dc.date.issued2014en_ZA
dc.description.abstractThe effects of pesticides on honeybee larvae are less understood than for adult bees, even though larvae are chronically exposed to pesticide residues that accumulate in comb and food stores in the hive. We investigated how exposure to a plant alkaloid, nicotine, affects survival, growth and body composition of honeybee larvae. Larvae of Apis mellifera scutellata were reared in vitro and fed throughout development on standard diets with nicotine included at concentrations from 0 to 1000 lg/100 g diet. Overall mortality across all nicotine treatments was low, averaging 9.8% at the prepupal stage and 18.1% at the white-eyed pupal stage, but survival was significantly reduced by nicotine. The mass of prepupae and white-eyed pupae was not affected by nicotine. In terms of body composition, nicotine affected water content but did not influence either protein or lipid stores of white-eyed pupae. We attribute the absence of consistent negative effects of dietary nicotine to detoxification mechanisms in developing honeybees, which enable them to resist both natural and synthetic xenobiotics.en_ZA
dc.description.sponsorshipBBSRC, NERC, the Wellcome Trust, Defra, and the Scottish Government under the Insect Pollinators Initiative (BB/I000968/1) National Research Foundation & University of Pretoriaen_ZA
dc.description.tableofcontentsThis xls file contains: • Colony data • Reference data • Lipid dry mass analyses • Proteinen_ZA
dc.identifier.citationHuman, H., Archer, R., du Rand, E.E. Pirk, C.W.W and Nicolson, S.W. (2014) Resistance of developing honeybee larvae during chronic exposure to dietary nicotine. Journal of Insect Physiology 69:74-79.en_ZA
dc.identifier.urihttp://hdl.handle.net/2263/49635
dc.language.isoenen_ZA
dc.publisherPretoria : University of Pretoria. Faculty of Natural and Agricultural Sciences. Department of Zoology & Entomologyen_ZA
dc.relation.isreferencedbydoi: 10.1016/j.jinsphys.2014.03.012en_ZA
dc.rights©University of Pretoria. Dept of Zoology & Entomology © C. Ruth Archer. Re-use permitted with attribution. See the suggested citation to this itemen_ZA
dc.rights.uri?en_ZA
dc.subjectApis mellifera scutellataen_ZA
dc.subjectIn vitro rearingen_ZA
dc.subjectLarval developmenten_ZA
dc.subjectLipidsen_ZA
dc.subjectPesticidesen_ZA
dc.subjectProteinen_ZA
dc.titleResistance of developing honeybee larvae during chronic exposure to dietary nicotineen_ZA
dc.typeDataseten_ZA

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