Biomolecular mechanism in the uptake of Pt(IV) by Saccharomyces cerevisiae EBY100 cells expressing platinum binding peptides

dc.contributor.authorMashangoane, Boitumelo Francina
dc.contributor.authorChirwa, Evans M.N.
dc.contributor.authorGidudu, Brian
dc.contributor.emailbmashangoane@tuks.co.za
dc.date.accessioned2025-09-30T11:53:44Z
dc.date.issued2024-08
dc.descriptionDATA AVAILBILITY : Data will be made available on request.
dc.description.abstractPlatinum group metals (PGMs) are currently under increased demand due to limited availability and their unique properties; therefore, their limited availability presents an economically attractive opportunity to recover these metals from waste. The recovery of PGMs from effluents using conventional techniques such floatation, precipitation and ion exchange results in large volumes of hazardous waste as well as high operating costs and low efficiencies. Biosorption using biomass such as microorganisms as adsorbents can offer a cheap, efficient and environmentally friendly alternative for the recovery of PGMs. Despite previous research on the use of biosorption techniques for the recovery of PGMs; there is still room for improvement in the way that microorganisms absorb metals. Such advancements can be achieved through the use of genetically engineered peptides for inorganics (GEPI) for cell surface display purposes. In this work, Saccharomyces cerevisiae EBY100 cells were genetically engineered for cell surface display of platinum binding peptides to enable Pt(IV) adsorption from an aqueous solution. The transformed Saccharomyces cerevisiae EBY100 cells attained the maximum adsorption of 40 % for Pt(IV) after 3 h at the optimal pH = 3. As the initial metal concentration rose from 10 ppm to 50 ppm, the biosorption Pt(IV) increased as well, reaching a biosorption capacity of 85 mg/g. The findings also showed that the Freundlich model best explained the Pt(IV) biosorption isotherm onto P3.1, while the pseudo-second-order model best explained the Pt(IV) adsorption kinetics onto P3.1. HIGHLIGHTS • Saccharomyces cerevisiae EBY100 cells express platinum binding peptides. • Genetically engineered Saccharomyces cerevisiae EBY100 cells adsorb Pt(IV). • Increase in initial Pt(IV) concentration increases biosorption capacity of the cells.
dc.description.departmentChemical Engineering
dc.description.embargo2026-06-08
dc.description.librarianhj2025
dc.description.sdgSDG-09: Industry, innovation and infrastructure
dc.description.sponsorshipRoyal Bafokeng Platinum.
dc.description.urihttps://www.elsevier.com/locate/mineng
dc.identifier.citationMashangoane, B.F., Chirwa, E.M.N. & Gidudu, B. 2024, 'Biomolecular mechanism in the uptake of Pt(IV) by Saccharomyces cerevisiae EBY100 cells expressing platinum binding peptides', Minerals Engineering, vol. 214, art. 108735, pp. 1-9, doi : 10.1016/j.mineng.2024.108735.
dc.identifier.issn0892-6875 (print)
dc.identifier.issn1872-9444 (online)
dc.identifier.other10.1016/j.mineng.2024.108735
dc.identifier.urihttp://hdl.handle.net/2263/104541
dc.language.isoen
dc.publisherElsevier
dc.rights© 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies. Notice : this is the author’s version of a work that was accepted for publication in Minerals Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Minerals Engineering, vol. 214, art. 108735, pp. 1-9, doi : 10.1016/j.mineng.2024.108735.
dc.subjectPlatinum group metals (PGMs)
dc.subjectBiosorption
dc.subjectGenetic engineering
dc.subjectBioconcentration
dc.subjectMinerals recovery
dc.subjectSaccharomyces cerevisiae EBY100
dc.titleBiomolecular mechanism in the uptake of Pt(IV) by Saccharomyces cerevisiae EBY100 cells expressing platinum binding peptides
dc.typePostprint Article

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