Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii

dc.contributor.authorVan de Vloet, Antoine
dc.contributor.authorProst-Boxoen, Lucas
dc.contributor.authorBafort, Quinten
dc.contributor.authorPaing, Yunn Thet
dc.contributor.authorCasteleyn, Griet
dc.contributor.authorJomat, Lucile
dc.contributor.authorLemaire, Stephane D.
dc.contributor.authorDe Clerck, Olivier
dc.contributor.authorVan de Peer, Yves
dc.date.accessioned2025-04-10T10:14:44Z
dc.date.available2025-04-10T10:14:44Z
dc.date.issued2025
dc.descriptionDATA AVAILABILITY : All haploid transformant strains (Table 1) will be made available through the Chlamydomonas Resource Center (https://www.chlamycollection.org/, Lefebvre et al., 2019). The strains will be available under the following cc nos.: H1, CC-6245 LEE-1; HygroR mt−; H2, CC-6246 LEE-3; ParoR mt+; H3, CC-6247 LEE-3; SpecR mt+; H4, CC-6248 LEE-3; BlastiR mt+; H5, CC-6249 Harris #6; HygroR mt−; H6, CC-6250 Harris #6; ParoR mt+; H7, CC-6251 Harris #6; SpecR mt+; H8, CC-6252 Harris #6; BlastiR mt+; H9, CC-6253 NIES-2464; HygroR mt+; H10, CC-6254 NIES-2464; ParoR mt+; H11, CC-6255 NIES-2464; SpecR mt+; H12, CC-6256 NIES-2464; and BlastiR mt+.en_US
dc.descriptionSUPPORTING INFORMATION : FIG. S1. Microscopic images of gamete cultures.en_US
dc.description.abstractWhole-genome duplications, widely observed in plant lineages, have significant evolutionary and ecological impacts. Yet, our current understanding of the direct implications of ploidy shifts on short- and long-term plant evolution remains fragmentary, necessitating further investigations across multiple ploidy levels. Chlamydomonas reinhardtii is a valuable model organism with profound potential to study the impact of ploidy increase on the longer term in a laboratory environment. This is partly due to the ability to increase the ploidy level. We developed a strategy to engineer ploidy in C. reinhardtii using noninterfering, antibiotic, selectable markers. This approach allows us to induce higher ploidy levels in C. reinhardtii and is applicable to field isolates, which expands beyond specific auxotroph laboratory strains and broadens the genetic diversity of parental haploid strains that can be crossed. We implement flow cytometry for precise measurement of the genome size of strains of different ploidy. We demonstrate the creation of diploids, triploids, and tetraploids by engineering North American field isolates, broadening the application of synthetic biology principles in C. reinhardtii. However, our newly formed triploids and tetraploids show signs of rapid aneuploidization. Our study greatly facilitates the application of C. reinhardtii to study polyploidy, in both fundamental and applied settings.en_US
dc.description.departmentBiochemistry, Genetics and Microbiology (BGM)en_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-15:Life on landen_US
dc.description.sponsorshipSorbonne Université; European Marine Biological Resource Centre Belgium; H2020 European Research Council; Bijzonder Onderzoeksfonds UGent; Fonds Wetenschappelijk Onderzoek.en_US
dc.description.urihttp://www.newphytologist.comen_US
dc.identifier.citationVan de Vloet, A., Prost-Boxoen, L., Bafort, Q. et al. 2025, 'Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii', New Phytologist, doi : 10.1111/nph.70095.en_US
dc.identifier.issn0028-646X (print)
dc.identifier.issn1469-8137 (online)
dc.identifier.other10.1111/nph.70095
dc.identifier.urihttp://hdl.handle.net/2263/101994
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation. This is the preprint version of the following article : 'Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii', New Phytologist, vol. , no. , pp. , 2025, doi : 10.1111/nph.70095. The definite version is available at : http://www.newphytologist.com.en_US
dc.subjectChlamydomonas reinhardtiien_US
dc.subjectExperimental modelen_US
dc.subjectFlow cytometryen_US
dc.subjectMatingen_US
dc.subjectPlant evolutionen_US
dc.subjectPolyploidyen_US
dc.subjectSynthetic ploidyen_US
dc.subjectTransformationen_US
dc.subjectSDG-15: Life on landen_US
dc.titleExpanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtiien_US
dc.typePreprint Articleen_US

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